Imaging of Hsp70-positive tumors with cmHsp70.1 antibody-conjugated gold nanoparticles

Imaging of Hsp70-positive tumors with cmHsp70.1 antibody-conjugated gold nanoparticles

Blog Article

Mathias K Gehrmann,1 Melanie A Kimm,2 Stefan Stangl,1 Thomas E Schmid,1 Peter B Noël,2 Ernst J Rummeny,2 Gabriele Multhoff11Department of Radiation Oncology, 2Department of Diagnostic and Interventional Radiology, Klinikum rechts der Isar, Technische Universität München, Munich, GermanyAbstract: Real-time imaging of small tumors is still one of the challenges in cancer diagnosis, prognosis, and monitoring of clinical outcome.Targeting canon imageclass mf227dw novel biomarkers that are selectively expressed on a large variety of different tumors but not normal cells has the potential to improve the imaging capacity of existing methods such as computed tomography.Herein, we present a novel technique using cmHsp70.1 monoclonal antibody-conjugated spherical gold nanoparticles for quantification of the targeted uptake of gold nanoparticles into membrane Hsp70-positive tumor cells.Upon binding, cmHsp70.

1-conjugated gold nanoparticles but not nanoparticles coupled to an isotype-matched IgG1 antibody or empty nanoparticles are rapidly taken up by radio birdman tshirt highly malignant Hsp70 membrane-positive mouse tumor cells.After 24 hours, the cmHsp70.1-conjugated gold nanoparticles are found to be enriched in the perinuclear region.Specificity for membrane Hsp70 was shown by using an Hsp70 knockout tumor cell system.Toxic side effects of the cmHsp70.

1-conjugated nanoparticles are not observed at a concentration of 1–10 µg/mL.Experiments are ongoing to evaluate whether cmHsp70.1 antibody-conjugated gold nanoparticles are suitable for the detection of membrane-Hsp70-positive tumors in vivo.Keywords: heat shock protein 70, tumor biomarker, theranostics, multimodal CT, multispectral CT, k-edge.